| | 1 | 2 |  Nadia Udalova  Mack Engbers Ashley Groothuis Rafael Bouts | U40 | 00:55:06 | |
| | 2 | 1 | Melle Bijstra Guido Bouwmeester Matthijs Hoppener Jurre Brandsen | U40 | 00:55:06 | |
| | 3 | 3 | Kay Wissink Raf Roefs Kim Evers Amber Brouwer | U40 | 00:55:29 | |
| | 4 | 4 | Mieke Beerens Nick Weerts Owen Keller Femke Van Bree | U40 | 00:56:56 | |
| | 5 | 1 | Bert Veenstra Mariska Vermeulen Marijke Van Den Brul Marnix Kaspers | 40+ | 00:57:08 | |
| | 6 | 2 | Lianne Van Woudenbergh David Blankenburgh Lonneke Van Peperstraten Richard Proost | 40+ | 00:59:39 | |
| | 7 | 5 | Jeroen Lindeboom Wessel Rietveld Eline Peterse Femke Herber | U40 | 01:00:59 | |
| | 8 | 3 | Kristie Kamphorst Jan Van Belzen Alexandra Jonkman Fulco Assendelft | 40+ | 01:02:40 | |
| | 9 | 4 | Elias Coxon Eveline Hadewegg Maita Vermeulen Omid Mehrani | 40+ | 01:04:17 | |
| | 10 | 6 | Freek Balk Alieke Carree Jarron Nederhoed Chantal Vernooij | U40 | 01:04:31 | |
| | 11 | 7 | Nick Mensink Quinie Verheijen Merel Sas Danny Tsang | U40 | 01:04:39 | |
| | 12 | 8 | Sam Lindeman Leonie Gotink Julian Van Der Bosch Julia Looman | U40 | 01:05:20 | |
| | 13 | 9 | Ilse Van Beusekom Jasper Avis Erik Saathoff Anne De Jong | U40 | 01:05:45 | |
| | 14 | 10 |  Nena Baijer Aarnav Singh Willow Portman Johannes Henkel | U40 | 01:07:16 | |
| | 15 | 11 | Sander Geerdink Patrick Reuvers Anouk Reuvers Margot Reuvers | U40 | 01:07:44 | |
| | 16 | 12 | Joost Van Der Have Danique Lemmers Fleur Toes Tim Maijers | U40 | 01:11:20 | |
| | 17 | 5 | Jody Salomon Suus Van Schadewijk Milou Salomon Lode Van Schadewijk | 40+ | 01:11:56 | |
| | 18 | 13 | Sanna Azzouz Patrick Philippa Daphne Kloosterman Wouter Hummelink | U40 | 01:12:15 | |
| | 19 | 14 | Sascha Baars Pauline Enschede Bram Hendrawan Made Diepersloot | U40 | 01:13:18 | |
| | 20 | 15 | Jesse Van Der Heijden Stina Sachse Jari Kroon Fenna Wüst | U40 | 01:13:40 | |
| | 21 | 6 | Rob Kruijssen Nicole Hermans Pascal Baetsen Katja America | 40+ | 01:13:48 | |
| | 22 | 16 |  Garv Khare Nadia Dos Santos Marlene De Pee Andres Perez | U40 | 01:14:11 | |
| | 23 | 17 |  Nusrath Khan Gareth Forbes  Anna Saville  Paula Zalaiskalna | U40 | 01:14:19 | |
| | 24 | 18 |  Melvin Heijmerikx Naomi De Olde Floris Terlou Anouk Van Eyck | U40 | 01:15:13 | |
| | 25 | 19 | Chantal Koch Marcel Vink Boy Berkelmans Sharon Nijenkamp | U40 | 01:15:32 | |
| | 26 | 7 | Rosaline Wedzinga Dave Frauenfelder Gerd De Smyter Henriette De Hoog | 40+ | 01:16:15 | |
| | 27 | 20 | Damien Van Mourik Kaja Sauren Lars Pethke Kirsten Bos | U40 | 01:17:19 | |
| | 28 | 21 |  Daniel Zito Lisette Beenders Tom Fleuren Kelsey Price | U40 | 01:18:24 | |
| | 29 | 22 | Tim Pronk Danielle Van Werkhoven Thimo Bakker Claudia Pawirosoerono | U40 | 01:20:24 | |
| | 30 | 8 | Stefan Honders Jacco Jordaan Josee Huisman Bianca Jordaan | 40+ | 01:20:43 | |
| | 31 | 23 | Keshav Kisoensingh  Samantha Figueroa Martijn Schooneman Shamiega Isaacs | U40 | 01:23:21 | |
| | 32 | 24 | Yury Delgado Morais Siedah Levant Rachel Bwatou Calvyn Stephens | U40 | 01:26:43 | |
| | 33 | 25 | Joyce Vrolijk Veerle Nuyens Van Nispen Thom Nuyens Edward Verhoeve | U40 | 01:27:47 | |
| | 34 | 26 | Robin Schoofs Liesbeth Hooghwerff Iris Langejans  Marc Bolto Gimo | U40 | 01:28:20 | |
| | 35 | 27 | Dirk Van Kampen Gabrielle Kortekaas Mark Rotteveel Regina Van Der Luit | U40 | 01:28:50 | |
| | 36 | 28 | Simon Epping Lisa Haverkort Fay Hooge Wesley Braker | U40 | 01:31:01 | |
| | 37 | 29 | Jeffrey Kiers Lobke Van Amersfoort Tooske Van Amersfoort Jeroen Van Amersfoort | U40 | 01:32:06 | |
| | 38 | 30 | Niels Van Der Zanden Lucas Van Der Zanden Bastienne Van Der Zanden - Bos Kirstin Van Der Zanden | U40 | 01:34:09 | |
| | 39 | 31 | Liza Moné - Van Der Linden Vincent Moné Yorick Wapstra Lisa-Marie Wapstra - Tax | U40 | 01:35:09 | |
| | 40 | 9 | Pim Hoes Mieke Visser Linda Slijk Frank Visser | 40+ | 01:52:10 | |
| | 41 | 32 | Louise Vink Joshua Vink Emilie Van Thiel  Davit Kordzaia | U40 | 01:53:05 | |
