| | 1 | 1 |  Dan Ratner Steph Ratner | 25-29 | 00:58:53 | |
| | 2 | 1 | Tanner Gregurich Shiloh Gregurich | 30-34 | 00:59:31 | |
| | 3 | 2 | Brett Johnson Lexi Johnson | 25-29 | 00:59:41 | |
| | 4 | 2 | McCall Hoyt  Matthew Grundy | 30-34 | 00:59:53 | |
| | 5 | 1 | Eric Bibb Reese Littlewood | 16-24 | 01:00:00 | |
| | 6 | 3 | Joseph Martello Cassandra Gill | 30-34 | 01:00:05 | |
| | 7 | 3 |  Sean Fraser Kaitlyn Colonna | 25-29 | 01:00:23 | |
| | 8 | 4 | Kiefer Calisto Kara VanDooijeweert | 30-34 | 01:00:29 | |
| | 9 | 1 | Dustin Needham Kim Stocklmeir | 40-44 | 01:00:38 | |
| | 10 | 4 | Elizabeth Burds Connor Weitz | 25-29 | 01:00:40 | |
| | 11 | 5 | Nicholas Karpiuk Lisa Rooke | 30-34 | 01:00:41 | |
| | 12 | 5 |  Juan Herrera  Vanessa Storaci | 25-29 | 01:00:48 | |
| | 13 | 2 | Nick Digani Liz Sheehan | 40-44 | 01:01:04 | |
| | 14 | 6 | Artem Kapshuk Rachael Bridgman | 30-34 | 01:01:12 | |
| | 15 | 1 | Renee Swider Justin Swider | 35-39 | 01:01:53 | |
| | 16 | 7 | Matt Mason Bella Stechschulte | 30-34 | 01:02:13 | |
| | 17 | 8 | Anna Christensen Alex Sroka | 30-34 | 01:02:23 | |
| | 18 | 3 |  Courtney France Jason France | 40-44 | 01:02:45 | |
| | 19 | 9 | Deana Jagielo Anthony Jagielo | 30-34 | 01:02:48 | |
| | 20 | 10 | Aaron Ordway Sydney Hudson | 30-34 | 01:02:59 | |
| | 21 | 6 | Brandon Orozco Ashley Lira | 25-29 | 01:03:30 | |
| | 22 | 2 | Lindsay Collins Ethan Goldman | 35-39 | 01:03:47 | |
| | 23 | 11 | Tricia Bolden Ashley Stephen | 30-34 | 01:04:02 | |
| | 24 | 4 | Alexander Valadja Meghan Morris | 40-44 | 01:04:43 | |
| | 25 | 1 | Sara Newton-Smith  David Kadio-Morokro | 45-49 | 01:04:50 | |
| | 26 | 12 | CeCe Cronin Dan Cronin | 30-34 | 01:04:51 | |
| | 27 | 2 | James-Ryan Salvi Lauren Hoffman | 16-24 | 01:04:52 | |
| | 28 | 7 | Emily Nikolai Tyler Finley | 25-29 | 01:04:58 | |
| | 29 | 13 | Joshua Herrington Kayla Christiansen | 30-34 | 01:05:05 | |
| | 30 | 2 | Jeffrey Wolschlag Krista Pufundt | 45-49 | 01:05:09 | |
| | 31 | 14 | Dan Nides Baylor McPherson | 30-34 | 01:05:29 | |
| | 32 | 8 | Megan Stuart Jack Hartigan | 25-29 | 01:05:31 | |
| | 33 | 9 | Kayla Jordan C.J Dick | 25-29 | 01:05:47 | |
| | 34 | 10 | Lindsey Blanks Samuel Humphries | 25-29 | 01:05:57 | |
| | 35 | 11 | Joshua Peterson Emma Diaz | 25-29 | 01:06:06 | |
| | 36 | 15 |  Sara Brumana Andrea Zoccali | 30-34 | 01:06:11 | |
| | 37 | 3 | Macy Corrona Spencer Corrona | 16-24 | 01:06:25 | |
| | 38 | 3 | Demetrios Mersinas Sophia Deslauriers | 35-39 | 01:06:31 | |
| | 39 | 4 | Noah Huyler Keri Herman | 16-24 | 01:06:38 | |
| | 40 | 12 | Jared Harrison Trista Pringle | 25-29 | 01:06:45 | |
| | 41 | 13 | Michael Goulard Miranda Wurtz | 25-29 | 01:06:55 | |
| | 42 | 5 | Allie Baker Cobe Huizenga | 16-24 | 01:06:56 | |
| | 43 | 16 | Madelaine Brown Jordan Molzan | 30-34 | 01:07:03 | |
| | 44 | 17 | Anna Willis John Reynolds | 30-34 | 01:07:04 | |
| | 45 | 18 | Avery Hohenstein Rachel Schwab | 30-34 | 01:07:08 | |
| | 46 | 19 | Danielle Berry Trey Berry | 30-34 | 01:07:10 | |
| | 47 | 4 | Kelly McClearn Bryan Musolf | 35-39 | 01:07:15 | |
| | 48 | 14 | Olivia Louthen Joe Blanchet | 25-29 | 01:07:16 | |
| | 49 | 6 | Addie Coppinger Spencer Derthick | 16-24 | 01:07:16 | |
| | 50 | 5 | Joshua Lambrecht Kenzie Evans | 35-39 | 01:07:17 | |
| | 51 | 15 | Mallory Harr Jalen Washington | 25-29 | 01:07:29 | |
| | 52 | 16 | Cody Walter Lexi Lutheran | 25-29 | 01:07:30 | |
| | 53 | 17 | Noah Strom Madysen Strom | 25-29 | 01:07:31 | |
| | 54 | 6 | yasmin tontat andrew tarleton | 35-39 | 01:07:31 | |
| | 55 | 21 | Haley Wallis Jared Lincoln | 30-34 | 01:07:40 | |
| | 56 | 20 |  Christopher Meyers Alexandra Meyers | 30-34 | 01:07:40 | |
| | 57 | 5 | ADRIENNE CHEN-OK Mykyta Yurtyn | 40-44 | 01:07:45 | |
| | 58 | 6 | Michael Garcia  Nastia Mederos | 40-44 | 01:07:56 | |
| | 59 | 7 | Derrick Brown Laura Edwards | 35-39 | 01:08:08 | |
| | 60 | 18 | Jayce Kuehnlein McKenzie Smith | 25-29 | 01:08:16 | |
| | 61 | 22 | Anna Baer Kelly Gibbons | 30-34 | 01:08:20 | |
| | 62 | 23 | Ian Gaffney Caroline Militello | 30-34 | 01:08:24 | |
| | 63 | 19 | Amanda Arendt Tristian Penick | 25-29 | 01:08:24 | |
| | 64 | 8 |  Rosimayri Hernandez Amaury Alvarez | 35-39 | 01:08:24 | |
| | 65 | 7 | Harrison Cowden Isabel Reisman | 16-24 | 01:08:38 | |
| | 66 | 24 | Christopher Goins  Rebeca Ponce Ochoa | 30-34 | 01:08:43 | |
| | 67 | 1 | David Miller Lara Mele | 50-54 | 01:08:43 | |
| | 68 | 25 | Ethan Mamuric Emily Coleman | 30-34 | 01:08:44 | |
| | 69 | 7 | Joe Miday Sarina Petroni-Miday | 40-44 | 01:08:50 | |
| | 70 | 9 | Sean Weckerly Hannah Bare | 35-39 | 01:08:55 | |
| | 71 | 10 | Franklin Mershon Jessie Mershon | 35-39 | 01:08:59 | |
| | 72 | 8 | Daniel Herman Megan Herman | 40-44 | 01:09:01 | |
| | 73 | 26 | Abigail Hoerchner Tarail King | 30-34 | 01:09:07 | |
| | 74 | 27 | Allison Shimer Tatsuya Takizawa | 30-34 | 01:09:12 | |
| | 75 | 28 |  Luis Calderon Leticia Garcia | 30-34 | 01:09:14 | |
| | 76 | 29 | Eric Bridgewater Heidi Bridgewater | 30-34 | 01:09:18 | |
| | 77 | 20 | Kirsten Andersson Ross Andersson | 25-29 | 01:09:20 | |
| | 78 | 21 | Maddie Geiger William Simpson | 25-29 | 01:09:21 | |
| | 79 | 30 | Sean Arney Kristen Arney | 30-34 | 01:09:22 | |
| | 80 | 31 | Erin Crawford Michael Crawford | 30-34 | 01:09:26 | |
| | 81 | 11 | Ann Marie Lindsley Rockefeller Smith | 35-39 | 01:09:29 | |
| | 82 | 12 | Timothy Stanis Sara White-Delehoy | 35-39 | 01:09:35 | |
| | 83 | 22 | Anders Olsen Maddy Kish | 25-29 | 01:09:35 | |
| | 84 | 23 | Erin Tyler Mike Tyler | 25-29 | 01:09:38 | |
| | 85 | 9 | Michael Meeks Jessica Cuddy | 40-44 | 01:09:42 | |
| | 86 | 10 | Andrew McNelis Katie Garcia | 40-44 | 01:09:43 | |
| | 87 | 11 | Dustin Diefenderfer Lindsey Diefenderfer | 40-44 | 01:09:45 | |
| | 88 | 13 | Nathan Miller Rachel Peterson | 35-39 | 01:09:45 | |
| | 89 | 24 | Emma Stratton Kyle Kempton | 25-29 | 01:09:48 | |
| | 90 | 25 | Ryan Henning Peyton Brueher | 25-29 | 01:09:54 | |
| | 91 | 32 | Chloe Kinsel Jered Sternaman | 30-34 | 01:09:55 | |
| | 92 | 26 | Ruston Matherne Kathryn Matherne | 25-29 | 01:09:56 | |
| | 93 | 12 | Rhonda Henderson Kenneth Henderson | 40-44 | 01:09:59 | |
| | 94 | 33 | Lindsay Horstmann Shaun Horstmann | 30-34 | 01:10:02 | |
| | 95 | 13 | Evan Blankenship Anina Blankenship | 40-44 | 01:10:03 | |
| | 96 | 34 | Angely Jimenez Nicholas Heyse | 30-34 | 01:10:06 | |
| | 97 | 27 | Micah Wassink Ryan Kim | 25-29 | 01:10:08 | |
| | 98 | 14 | Kevin Lora Mandi Lora | 35-39 | 01:10:14 | |
| | 99 | 3 | Marcus Malcolm Rebekah Greer | 45-49 | 01:10:19 | |
| | 100 | 14 | Dana Thomas Jacob Thomas | 40-44 | 01:10:20 | |
