| 1 |  Alexis Dreiss Gabriela Olivier Jose Carchi Sam Morris | U40 | 03:50 U40 | |
| 2 | | U40 | 03:51 U40 | |
| 3 | Carla Rodrigues Michele Litchman Pat La Neve Rhudson Fonseca | U40 | 04:02 U40 | |
| 4 | Anita Girdhari Harold Varah Natalie Orozco Ruben Belliard | 40+ | 04:07 40+ | |
| 4 | Andie Knox Chris Frank Lizzy Carson Rob Carson | 40+ | 04:07 40+ | |
| 6 | Christian Mendez Hugo Osorio Julianne Farewell Samantha Lennon | U40 | 04:12 U40 | |
| 7 | Daniel London Maribel Mazabuel Rivera Nicole Scibelli Tyrone London | U40 | 04:14 U40 | |
| 8 | Amanda Olgren Ashley Wyatt Kevin Downing Scott Mcghee | U40 | 04:23 U40 | |
| 8 | Alissa Jones Johnny Garcia Nirav Doshi Pearl Amin | U40 | 04:23 U40 | |
| 10 | Amanda Factor Joseph Matta Keren Salem-Tejada Matt Sisneros | U40 | 04:27 U40 | |
| 11 | Arnold Situmeang Rachael Libertin Ronnie Mewengkang Torrye Zullo | U40 | 04:32 U40 | |
| 12 | Ashley Macha Dan Conroy Matt Merenich Stephanie Arabagian | U40 | 04:35 U40 | |
| 12 | Chase Meader Jeff Matthews Lauren Hsu Su Meader | U40 | 04:35 U40 | |
| 14 | Bruce Eckel Danielle Mrosko Gabby Turner Joseph Jaworski | 40+ | 04:40 40+ | |
| 15 | Heidi Jones Lauren Foundos Matilda Dingemans Toby Dingemans | 40+ | 04:46 40+ | |
| 16 |  Jin Im Brancalhao Jin Stephens Jin Yang Brancalhao Kimiko Gomi | U40 | 04:53 U40 | |
| 17 | Blake Balda Jillian Carter Julia Wilson Thomas Diop | U40 | 04:59 U40 | |
| 18 | Ashanti Ehrhart Breanna Hall Finzo Hall Keith Ehrhart | U40 | 05:35 U40 | |
| 19 | Catalina Ruiz Julie Silva Patricia Baile Suzanne Christy | 40+ | 05:40 40+ | |
| 20 | Jarrett Levin Klitos Teklos Or Artzi Skylar Davis | U40 | 05:41 U40 | |
| 21 | Caroline Lee Dlan Rush Lawrence Montgomery Meg Stier | U40 | 05:51 U40 | |
| 22 | David Kadio-Morokro Regina Dioniso Rosie Chen Tom Goforth | 40+ | 06:28 40+ | |
