| | 1 | 1 |  Raf Roefs Fleur Van Dijk Amber Brouwer Brian Vogelzang | U40 | 00:54:08 | |
| | 2 | 2 |  Nerea Izcue  Callum Li  Stefan Pecelj Yemisi Edgar | U40 | 00:55:46 | |
| | 3 | 3 |  Carolin Weihrauch Thea Fliegauf Robert Gutknecht David Flaig | U40 | 00:58:10 | |
| | 4 | 4 |  Gianmarco Carra Ludovica Midei Tommaso Appignanesi Fabrizia Midei | U40 | 00:58:26 | |
| | 5 | 5 | Gaetano Fidanzati Veronica Paladino Andrea Orlacchio Lucia Dolce | U40 | 00:59:24 | |
| | 6 | 6 |  Domonkos Kővári Balázs Gyenes Léda Rebeka Kővári Tekla Sary | U40 | 00:59:37 | |
| | 7 | 7 | Matteo Furlan Claudia Pontoglio Pamela Bodei Paolo Denti | U40 | 01:06:04 | |
| | 8 | 1 |  Brian Rappert  Kirsten Walsh  Adrian Currie  Emile Barberon | 40+ | 01:06:58 | |
| | 9 | 8 | Abramo Zancanella Michela Perazzolo Marina Perazzolo Marco Dal Pra' | U40 | 01:07:06 | |
| | 10 | 9 | Marco Maria Manfredi Andrea Frascoli Antonia Canini Anna Lugoboni | U40 | 01:08:04 | |
| | 11 | 10 | Miryea Stabile Francesca Silva Ithocor Meloni Rosario Internullo | U40 | 01:09:22 | |
| | 12 | 11 | Marta Atzori Lorenzo Liguori Carlo Fiorentini Vittoria Benedetti | U40 | 01:09:30 | |
| | 13 | 12 | Anna-Lena Rösler Max Bolzen Holger Kunzmann Sophia Baur | U40 | 01:10:10 | |
| | 14 | 2 | Virág Rita Kővári-Varga Zsófia Pázmándi István Kis Levente Bernáth | 40+ | 01:11:12 | |
| | 15 | 13 |  Asmae Ajana  kawtar zahi YOUNES MELLAL otmane el manser | U40 | 01:12:32 | |
| | 16 | 14 | Nicole Martini Nicoletta Previdi Filippo Furnari Jacopo Maragna | U40 | 01:12:34 | |
| | 17 | 15 | Giovanni Fois Federico Leo Laura Salvetti Federica Donadio | U40 | 01:13:01 | |
| | 18 | 16 |  Tadeáš Pospíšil Andrew Seffi Gabriela Juříčková Alice Herodesová | U40 | 01:13:02 | |
| | 19 | 17 | Marco Quattrociocchi Mario Reali Miriam Iannarilli Giorgia Paolucci | U40 | 01:13:13 | |
| | 20 | 18 | Riccardo Preziati Mattia Costa Greta Lenti Alessia Lecchi | U40 | 01:13:21 | |
| | 21 | 19 | Vanessa Parietti Andrea Turco Sara Crespi Valerio D’Acunto | U40 | 01:13:24 | |
| | 22 | 20 | Alice Veghini Carlalberto Francia Lorenzo Riccò Giulia Menozzi | U40 | 01:13:46 | |
| | 23 | 21 | Guillaume Machado Salomé Botas Mendes Romane Niopel Alexandre Tuffin | U40 | 01:13:48 | |
| | 24 | 22 | Alice Minotti Alex Miglietta Lidia Miglietta Luca Papi | U40 | 01:17:14 | |
| | 25 | 23 | Valentina Bassi Giovanni D’Agostino Chiara Raucci Nicholas Gesuele | U40 | 01:19:52 | |
| | 26 | 24 | MATTIA FALDUTO MASSIMILIANO FALCO FRANCESCA PEREGO ELEONORA MEAZZI | U40 | 01:20:46 | |
| | 27 | 25 | Davide Martinelli Ivan Gurrieri Amanda Ciraci Emanuela Lauretta | U40 | 01:22:12 | |
| | 28 | 26 | Andrea Cianchi Camilla Bianchi Simone Paggetti Francesca Nardi | U40 | 01:23:01 | |
| | 29 | 27 | Alessandra Daretti Erik Bruson Aris Dedini Elisa Negrini | U40 | 01:25:53 | |
| | 30 | 28 | Davide Petronio Andrea Esposito Francesca Rita Feltrin Eleonora Carlotta Brina | U40 | 01:26:28 | |
| | 31 | 29 | Matilde Trocchi Edoardo Trocchi Ida Domenicali Ettore Domenicali | U40 | 01:30:28 | |
| | 32 | 30 | Anna Mettifogo Silvia Betti Emanuel Mihali Marco Domenico Santambrogio | U40 | 01:38:11 | |
| | 33 | 31 | Melissa Jones James Morris Jones II Jason Jones Ashley Nesbitt Jones | U40 | 01:43:56 | |
