| 1 |  Liam McGuire Sophia Jagoe-Seidl | 25-29 | 03:49 25-29 | |
| 2 | Anna Foley Jack Klopfenstein | 25-29 | 03:50 25-29 | |
| 3 | Carly Pederson Kenneth Henderson | 35-39 | 03:54 35-39 | |
| 3 | Mekenzie Riley Roy Riley | 35-39 | 03:54 35-39 | |
| 5 | Jared Lawrence Melody Berrong | 40-44 | 03:56 40-44 | |
| 5 | Kate Brownell Kevin Gregory | 35-39 | 03:56 35-39 | |
| 5 | Sydney Smith Tyler Cooke | 25-29 | 03:56 25-29 | |
| 8 | Austin Miele Maddy Kenyon | 30-34 | 03:57 30-34 | |
| 8 | DJ Stover Emily Bly | 30-34 | 03:57 30-34 | |
| 8 | Blakeney Sitton Jake Axman | 35-39 | 03:57 35-39 | |
| 11 | James Barber Moriah Barber | 25-29 | 03:58 25-29 | |
| 12 | Bailey Cook Davey Fernandez | 30-34 | 03:59 30-34 | |
| 12 | Dominique Sottile Matthew Scrivner | 25-29 | 03:59 25-29 | |
| 14 | Megan Hendricks Zach Sattler | 25-29 | 04:00 25-29 | |
| 14 | John Cowden Sarah Cowden | 30-34 | 04:00 30-34 | |
| 14 | Corey Davis Kellyn Bowden | 35-39 | 04:00 35-39 | |
| 17 | Nicole Carter Ryan Maguire | 30-34 | 04:01 30-34 | |
| 17 | LaLo Contreras Nikki Bruno | 30-34 | 04:01 30-34 | |
| 17 | Jenna Littmann Kiefer Calisto | 30-34 | 04:01 30-34 | |
| 17 | Alyssa Farmer Gavin Farmer | 16-24 | 04:01 16-24 | |
| 17 | Michael Nail Sarah Nail | 35-39 | 04:01 35-39 | |
| 17 | Luke Bishop Morgan Davis | 35-39 | 04:01 35-39 | |
| 23 |  Leila Rizek  Luis Gonzalez | 40-44 | 04:02 40-44 | |
| 24 | Kaitlyn Findlay Kelly Findlay | 30-34 | 04:03 30-34 | |
| 24 | Lauren Thompson Michael Baker | 16-24 | 04:03 16-24 | |
| 26 | Lucas Warfield Molly Chambers | 16-24 | 04:04 16-24 | |
| 26 | Jesse Littlefield Kailey Littlefield | 30-34 | 04:04 30-34 | |
| 26 | Shi Ling Hu Tyson King | 30-34 | 04:04 30-34 | |
| 26 | John Vann  Kristina Vujanic | 30-34 | 04:04 30-34 | |
| 30 | Ester Kim Hunter Lainhart | 35-39 | 04:06 35-39 | |
| 30 | Kevin Lora Mandi Lora | 35-39 | 04:06 35-39 | |
| 30 | Alison Kucharik Michael Kucharik | 30-34 | 04:06 30-34 | |
| 30 | Camille Caldwell Nelly J | 25-29 | 04:06 25-29 | |
| 30 | Jaci Caceres Talon Smith | 40-44 | 04:06 40-44 | |
| 35 |  Jess Lancaster Reece Fallon | 30-34 | 04:07 30-34 | |
| 35 | Jarred East Meghan Urevick | 30-34 | 04:07 30-34 | |
| 35 | Chelsea Riley Kolby Watt | 35-39 | 04:07 35-39 | |
| 35 | Brittany George Jackson Ward | 16-24 | 04:07 16-24 | |
| 35 | Kameron Smith Stephanie Hahn | 25-29 | 04:07 25-29 | |
| 35 | Melissa Good Rafal Skos | 40-44 | 04:07 40-44 | |
| 41 | Hayden Frederick Jose Blankenship | 25-29 | 04:08 25-29 | |
| 41 | James Towner Maggie Mahla | 40-44 | 04:08 40-44 | |
| 41 | Adrian Bosch Jazmine Prada | 30-34 | 04:08 30-34 | |
| 41 | Andre Gathers  Lia Willis | 25-29 | 04:08 25-29 | |
| 41 | Daniel Lampl Katie Lampl | 35-39 | 04:08 35-39 | |
| 41 | Angela Hochhalter Brent Denson | 30-34 | 04:08 30-34 | |
| 41 | Jack Odom Stacey Odom | 35-39 | 04:08 35-39 | |
| 41 | AJ Gill Jordan Brisson | 30-34 | 04:08 30-34 | |
| 41 | Cali Hancock Zachary Hancock | 30-34 | 04:08 30-34 | |
| 41 | Jeffrey Colquitt  Karen Valera | 30-34 | 04:08 30-34 | |
| 51 | Ansley Oliver Mitchell Clegg | 25-29 | 04:09 25-29 | |
| 51 |  Samet Isleyen Suzie Buchanan | 35-39 | 04:09 35-39 | |
| 51 | Anna Gill Davis Gill | 25-29 | 04:09 25-29 | |
| 54 | Ellie Kearney Smith Caudell | 16-24 | 04:10 16-24 | |
| 54 | Mike Holliman Tori Wheatley | 30-34 | 04:10 30-34 | |
| 54 | Courtney Meadows Oluwatosin Komolafe-Williams | 25-29 | 04:10 25-29 | |
| 54 | Cori Bradham Robert Bradham | 30-34 | 04:10 30-34 | |
| 58 | Athena McDowall Joseph Anzur | 25-29 | 04:11 25-29 | |
| 58 | Andrew Siegelin Corinne Siegelin | 16-24 | 04:11 16-24 | |
| 58 | Eric Hutterer Judika Timar-Hutterer | 40-44 | 04:11 40-44 | |
| 58 | Hilary Gerrish Tim Gerrish | 40-44 | 04:11 40-44 | |
| 58 | Margaret Hellwig Michael Luckie | 16-24 | 04:11 16-24 | |
| 58 | Kristen Hepler Kyle Zoeller | 25-29 | 04:11 25-29 | |
| 58 | Chase Reeves Kate Reeves | 40-44 | 04:11 40-44 | |
| 65 | Connor Walpole Liz Walpole | 25-29 | 04:12 25-29 | |
| 65 | Andrew Clapper Kathryn Maloof | 30-34 | 04:12 30-34 | |
| 67 | Christopher Balven Kara Mobly | 35-39 | 04:13 35-39 | |
| 67 | Katherine Hansen Nick Provo | 35-39 | 04:13 35-39 | |
| 67 | Amanda Fischer Thomas Carroll | 30-34 | 04:13 30-34 | |
| 67 | Julie Smith Rob Brock | 45-49 | 04:13 45-49 | |
| 67 | Justyn Solomon Lauren Noble | 25-29 | 04:13 25-29 | |
| 67 | Beccy Smith Chris Smith | 40-44 | 04:13 40-44 | |
| 67 | Grant Geier Rylee Geier | 16-24 | 04:13 16-24 | |
| 67 | Josh Ellard Maddie McGriff | 25-29 | 04:13 25-29 | |
| 67 | Andy Yalung  Pip Clarke | 25-29 | 04:13 25-29 | |
| 76 |  Joe Rivera Sarah Hernandez | 50-54 | 04:14 50-54 | |
| 76 |  Diego Bravo Palafox Sarah Easterling | 30-34 | 04:14 30-34 | |
| 76 | Allie Weiss Michael Weiss | 25-29 | 04:14 25-29 | |
| 76 | Jesse Kurkulonis Maggie McWilliams | 16-24 | 04:14 16-24 | |
| 76 | Alexander Fraser Jenna Benton | 35-39 | 04:14 35-39 | |
| 76 | Erin Green Logan McAllister | 30-34 | 04:14 30-34 | |
| 76 | Amanda Woods Micheal Woods | 30-34 | 04:14 30-34 | |
| 83 | Miricle Corbo Zayren Terukina | 25-29 | 04:15 25-29 | |
| 83 | Bianca Quisenberry Ralph David Abernathy IV | 30-34 | 04:15 30-34 | |
| 83 | Caitlin Beacom Ryan Creecy | 25-29 | 04:15 25-29 | |
| 83 | Chace Blaylock Sydney Harris | 30-34 | 04:15 30-34 | |
| 87 | Daniel Lee Mallory Ortman | 40-44 | 04:16 40-44 | |
| 87 | Nicole Roa Trea Futral | 25-29 | 04:16 25-29 | |
| 87 | Patrick Gartner Sophie Katz | 16-24 | 04:16 16-24 | |
| 87 | Austin Harris Watson Harris | 30-34 | 04:16 30-34 | |
| 87 | Jade King  Simon Bratke | 30-34 | 04:16 30-34 | |
| 87 | Ashley Sheard  Wesley Sheard | 40-44 | 04:16 40-44 | |
| 87 | Alex Jolley Courtney Nicholson | 35-39 | 04:16 35-39 | |
| 87 | Collin Weber Julie Lundgren | 35-39 | 04:16 35-39 | |
| 87 | Onalee Devereaux Steven Walker | 30-34 | 04:16 30-34 | |
| 87 | Bryson Tolley Faith Reid | 25-29 | 04:16 25-29 | |
| 87 | Megan Roberts Zach Bull | 35-39 | 04:16 35-39 | |
| 98 | Amber Catalioto Martin Catalioto | 40-44 | 04:17 40-44 | |
| 98 | Jonathan Newell Mary Delatte | 40-44 | 04:17 40-44 | |
| 98 | Garrett Glover Katie Glover | 25-29 | 04:17 25-29 | |
