| 1 |  Cynthia Ileso Marcellis Wade Victor Umeh Xochitl Alvarez | U40 | 02:48 U40 | |
| 2 | Jessica Sarni Kaleb Yates Keaton Nelligan Taylor Roberts | U40 | 03:50 U40 | |
| 2 | Brianna Warren Chinweuba Nkenke Lex Poole Mykal Hogue | U40 | 03:50 U40 | |
| 4 | Christopher Balven Juan Herrera Kara Mobly  Maria Fecik | U40 | 03:51 U40 | |
| 5 | Allison Motto Britton Ezell Conner Netherland Mallory Meredith | U40 | 04:02 U40 | |
| 6 | Daniel Ochoa Nicki Forte Robert Lee Stephanie Arreola-Perez | U40 | 04:04 U40 | |
| 6 | Amy Harenberg Ashlee James George Blevins  Yeicob Calderon | U40 | 04:04 U40 | |
| 8 | Alicia Kueker Dave Martinsky Dave Summerton Haley Brogan | U40 | 04:05 U40 | |
| 9 | Hajime Ichikawa Jenna Littmann Kayla Cittadino Kiefer Calisto | U40 | 04:07 U40 | |
| 10 | Chase Garsee Jenny Lawson Josh Lawson Raegen Martin | U40 | 04:17 U40 | |
| 11 | Megan May Ryan Moody Sara Ahmad Tom Wilson | 40+ | 04:24 40+ | |
| 11 | Erin Morris Logan Hayter Monica Jangrus Ray Morris | 40+ | 04:24 40+ | |
| 13 | Jordan Babcock Madison Valdez Maggie Babcock Will Valdez | U40 | 04:27 U40 | |
| 14 | Jason Blackwood Joe Chiavone Katherine Chiavone Mariah Smith | U40 | 04:30 U40 | |
| 14 | Heather Liner John Krueger John Wallet Lauren Miller | 40+ | 04:30 40+ | |
| 16 | Bridget Hillseth Cathy Hensley Don Rebsamen Hernaniz Herrera | U40 | 04:31 U40 | |
| 17 |  Clara Nolasco  Katherine Bermudez Mario Bermudez  Marvin Rodriguez | U40 | 04:35 U40 | |
| 18 | Debrenda Nash Greg Guse Laura Derks Vernell Robinson | U40 | 04:40 U40 | |
| 19 | Curtis Okafor Devyn Oatis Jasmin Thompson Victoria Adesanya | U40 | 04:41 U40 | |
| 20 | Allison Corona Del Cid  Edward Mitri Jake Lundy Zophia Corona Del Cid | U40 | 04:42 U40 | |
| 21 | Jesus Cadena Kelsey Thompson Molly Walton Nubia Millan | U40 | 04:49 U40 | |
| 22 | Amanda Alexander Cassie Mcclure Gabriel Garza Noah Islas | U40 | 04:50 U40 | |
| 23 | | — | 04:55 | |
| 23 | Blaine Blackburn Brooke Smith Lauren Babin Mitch Scarmardo | U40 | 04:55 U40 | |
| 23 | Emme Rhodes Jackson Malcolm Morgan Powell Saxon Stroud | U40 | 04:55 U40 | |
| 26 | Daniel Cabungcal Diana Vila Heather Horsley Will Mayes | U40 | 05:01 U40 | |
| 27 | Courtney Snukis Jacob Batchelor Kristina Boyle Robert Goodin | U40 | 05:07 U40 | |
| 27 | Kimberly Senni Mark Schoenemann Peter Neang Sophia Neang | U40 | 05:07 U40 | |
| 29 | Judge Hansen Paige Crawford Peyton Ford  Valentina Michelena | U40 | 05:08 U40 | |
| 30 |  Alli Yost Camille Shaw Joshua Sanders Richard Mann | U40 | 05:09 U40 | |
| 30 | Garrett Hite Grant Weller Madison Carter Victoria Poplin | U40 | 05:09 U40 | |
| 32 | Amaury Collado Jeff Polding Lori Collado Mary Polding | 40+ | 05:12 40+ | |
| 33 | Caroline O'Driscoll  Jeremy Rodriguez Kole Boucher Mary Worth | U40 | 05:13 U40 | |
| 34 | Julio César Ontiveros Jr Julio Ontiveros Teresa Ontiveros Veronica Ontiveros | U40 | 05:14 U40 | |
| 35 | Ella Horne  Hershell Harvey Linton Brown Tavia Garvey | U40 | 05:21 U40 | |
| 36 | Andrea Carrasco Josh Carrasco  Steven Gunawan Tulia Taborga-Urquiola | U40 | 05:24 U40 | |
| 37 | | — | 05:26 | |
| 38 | Ana Alvarez Jake Munta Michael Warber Vanessa Murrell | 40+ | 05:28 40+ | |
| 38 | Jadira Jazmine Varela Lynn Notarianni Miguel Millan Rolando Celis | U40 | 05:28 U40 | |
| 40 | Kweku Arkorful Natalie Thomas Rob Montealvo Sarah Mumma | U40 | 05:39 U40 | |
| 41 | Ashley Golemon Rachel Wood STEVEN SIMPSON Tyler Smith | U40 | 05:42 U40 | |
| 42 | Cameron Dean Grant Bergeron Heather Bush Meghan Malone | U40 | 05:47 U40 | |
| 42 | Anna Stevens Chuck Goff Jose Moreno Lauren Reed | U40 | 05:47 U40 | |
| 44 | Angela Guffey Bryce Guffey Kyle Barden Rachel Barden | U40 | 05:48 U40 | |
| 44 |  Monika Veleva Peter Mantaring Philip Mantaring Sue Covert | 40+ | 05:48 40+ | |
| 46 | Emily Hu Eric Hu  Marc Seah Mi Rae Lee | U40 | 05:51 U40 | |
| 47 | Delta Gutierrez-Garcia Edward Irizzary Gabriela Navarrete Jason Adams | 40+ | 06:08 40+ | |
| 48 | Jose Vega Scott Wilson Valerie Wilson Vanessa Gonzalez | 40+ | 06:09 40+ | |
| 49 | Charissa Stewart Christine Rand Jonathan Arrington Ricky Chatman | U40 | 06:11 U40 | |
| 50 | Benjamin Darbe Cristina Patton Douglas Smith Sydney Darbe | U40 | 06:16 U40 | |
| 50 | Glad Nwaozo Kingsley Nwaozo Trenton Orr Vanessa Nzeh | U40 | 06:16 U40 | |
| 50 | Douglas Williams Greta Cambridge Jason Leslie Sheila Willis | 40+ | 06:16 40+ | |
| 50 | Albert Valverde Janell Gambrell Nicolas Arredondo Sharon Joseph | U40 | 06:16 U40 | |
| 54 | Brook Varner Jason Suber Keesha Partida Mark De Leon | U40 | 06:30 U40 | |
| 55 | Jennifee Fordyce Jim Fordyce Justin Fordyce Kooper Briley | 40+ | 06:39 40+ | |
| 56 | Joshua Adesanya Mary Walker  Nana Thompson Vivian Adesanya | U40 | 06:46 U40 | |
